A Conserved Metal Binding Motif in the Bacillus subtilis Competence Protein ComFA Enhances Transformation
نویسندگان
چکیده
منابع مشابه
Development of competence in the Bacillus subtilis transformation system.
Competence in Bacillus subtilis, assayed by the ability of cells to be transformed with bacterial deoxyribonucleic acid (DNA) or transfected by phage DNA, has been shown to occur in a single semisynthetic medium with peak activity occurring 3 hr after the cessation of logarithmic growth. No step-down conditions or culture manipulations were necessary for routine transfection of 1% of the popula...
متن کاملBiochemical Aspects of Competence in the Bacillus subtilis Transformation System
A lytic factor, associated with the cell wall, was fortuitously discovered during the course of the investigations described in the previous paper (1). In the pneumococcal transformation system (2-5) as well as in the Bacillus subtilis transformation system (6), the highly transformable strains possessed active autolytic enzymes. Consequently, studies were undertaken to investigate the relation...
متن کاملThe conserved DNA-binding protein WhiA is involved in cell division in Bacillus subtilis.
Bacterial cell division is a highly coordinated process that begins with the polymerization of the tubulin-like protein FtsZ at midcell. FtsZ polymerization is regulated by a set of conserved cell division proteins, including ZapA. However, a zapA mutation does not result in a clear phenotype in Bacillus subtilis. In this study, we used a synthetic-lethal screen to find genes that become essent...
متن کاملCharacterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis
HutP is an RNA-binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on hut mRNA. It requires L-histidine and an Mg2+ ion for binding to the specific sequence within the hut mRNA. In the present study, we show that several divalent cations can mediate the HutP-RNA interactions. The best divalen...
متن کاملTransformation in Bacillus subtilis: involvement of the 17-kilodalton DNA-entry nuclease and the competence-specific 18-kilodalton protein.
A protein complex, consisting of a 17-kilodalton (kDa) nuclease and an 18-kDa protein, is believed to be involved in the binding and entry of donor DNA during transformation of Bacillus subtilis (H. Smith, K. Wiersman, S. Bron, and G. Venema, J. Bacteriol. 156:101-108, 1983). In this paper, the nucleotide sequences of the genes encoding both the nuclease and the 18-kDa protein are presented. Th...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2017
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00272-17